Enzyme-linked Immunosorbent Assay:Article Title: Modulation function of sphingomyelin molecular species in TLR4 signaling and cell death.
Article Snippet: The obtained bone marrow cells were washed in DMEM and cultured for 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL recombinant mouse Granulocyte-macrophage colony-stimulating factor (GM-CSF) (BioLegend) and IL4 (BioLegend) to differentiate into BMDC or, cultured for .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays ELISA MAX Deluxe Set Mouse IL-1α BioLegend 433404 ELISA MAX Deluxe Set Mouse IL-1β BioLegend 432604 Mouse IL-6 ELISA Kit Invitrogen BMS603-2 Human IL-6 ELISA Kit Invitrogen EH2IL6 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3TEN Pierce TM BCA Protein Assay Kits Thermo Fisher Scientific 23227 Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs Bio-Rad 1704156 Cell Counting Kit-8 Dojindo CK04 Cytotoxicity LDH Assay Kit-WST Dojindo CK12 Experimental models: Cell lines Raw264.7 ATCC TIB-71 THP-1 JCRB Cell Bank JCRB0112 HEK-Blue TM hTLR4 InvivoGen Cat.code: hkb-htlr4 HEK-Blue TM mTLR4 InvivoGen Cat.code: hkb-htlr4 RAW-ASC-WT InvivoGen Cat.code: raw-asc RAW-ASC-Caspase11-KO InvivoGen Cat.code: raw-kocasp11 RAW-ASC-GSDMD-KO InvivoGen Cat.code: raw-kogsdmd HEK293T ATCC CRL-3216 AD293 Agilent Technologies #240085 HeLa JCRB Cell Bank JCRB9004 Recombinant DNA pET-BA BioDynamics Laboratory Inc. DV210 Halotag ORF Clone pFN21AE1334 Kazusa DNA Research Institute FHC08921 Software and algorithms ImageJ Schneider et al. 32 https://imagej.nih.gov/ij/ GraphPad Prism 10 GraphPad https://www.graphpad.com/features Flowjo BD https://www.flowjo.com/ BioRender BioRender https://www.biorender.com/ AutoDock Vina Version 1.2.7 The Center for Computational Structural Biology (CCSB) https://vina.scripps.edu/ PyMOL DeLano Scientific LLC https://www.pymol.org/ 18 Cell Reports 44, 116568, December 23, 2025 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL Macrophage colony stimulating factor (M-CSF) (BioLegend) to differentiate into BMDM. ..
Bicinchoninic Acid Protein Assay:Article Title: Modulation function of sphingomyelin molecular species in TLR4 signaling and cell death.
Article Snippet: The obtained bone marrow cells were washed in DMEM and cultured for 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL recombinant mouse Granulocyte-macrophage colony-stimulating factor (GM-CSF) (BioLegend) and IL4 (BioLegend) to differentiate into BMDC or, cultured for .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays ELISA MAX Deluxe Set Mouse IL-1α BioLegend 433404 ELISA MAX Deluxe Set Mouse IL-1β BioLegend 432604 Mouse IL-6 ELISA Kit Invitrogen BMS603-2 Human IL-6 ELISA Kit Invitrogen EH2IL6 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3TEN Pierce TM BCA Protein Assay Kits Thermo Fisher Scientific 23227 Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs Bio-Rad 1704156 Cell Counting Kit-8 Dojindo CK04 Cytotoxicity LDH Assay Kit-WST Dojindo CK12 Experimental models: Cell lines Raw264.7 ATCC TIB-71 THP-1 JCRB Cell Bank JCRB0112 HEK-Blue TM hTLR4 InvivoGen Cat.code: hkb-htlr4 HEK-Blue TM mTLR4 InvivoGen Cat.code: hkb-htlr4 RAW-ASC-WT InvivoGen Cat.code: raw-asc RAW-ASC-Caspase11-KO InvivoGen Cat.code: raw-kocasp11 RAW-ASC-GSDMD-KO InvivoGen Cat.code: raw-kogsdmd HEK293T ATCC CRL-3216 AD293 Agilent Technologies #240085 HeLa JCRB Cell Bank JCRB9004 Recombinant DNA pET-BA BioDynamics Laboratory Inc. DV210 Halotag ORF Clone pFN21AE1334 Kazusa DNA Research Institute FHC08921 Software and algorithms ImageJ Schneider et al. 32 https://imagej.nih.gov/ij/ GraphPad Prism 10 GraphPad https://www.graphpad.com/features Flowjo BD https://www.flowjo.com/ BioRender BioRender https://www.biorender.com/ AutoDock Vina Version 1.2.7 The Center for Computational Structural Biology (CCSB) https://vina.scripps.edu/ PyMOL DeLano Scientific LLC https://www.pymol.org/ 18 Cell Reports 44, 116568, December 23, 2025 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL Macrophage colony stimulating factor (M-CSF) (BioLegend) to differentiate into BMDM. ..
CCK-8 Assay:Article Title: Modulation function of sphingomyelin molecular species in TLR4 signaling and cell death.
Article Snippet: The obtained bone marrow cells were washed in DMEM and cultured for 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL recombinant mouse Granulocyte-macrophage colony-stimulating factor (GM-CSF) (BioLegend) and IL4 (BioLegend) to differentiate into BMDC or, cultured for .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays ELISA MAX Deluxe Set Mouse IL-1α BioLegend 433404 ELISA MAX Deluxe Set Mouse IL-1β BioLegend 432604 Mouse IL-6 ELISA Kit Invitrogen BMS603-2 Human IL-6 ELISA Kit Invitrogen EH2IL6 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3TEN Pierce TM BCA Protein Assay Kits Thermo Fisher Scientific 23227 Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs Bio-Rad 1704156 Cell Counting Kit-8 Dojindo CK04 Cytotoxicity LDH Assay Kit-WST Dojindo CK12 Experimental models: Cell lines Raw264.7 ATCC TIB-71 THP-1 JCRB Cell Bank JCRB0112 HEK-Blue TM hTLR4 InvivoGen Cat.code: hkb-htlr4 HEK-Blue TM mTLR4 InvivoGen Cat.code: hkb-htlr4 RAW-ASC-WT InvivoGen Cat.code: raw-asc RAW-ASC-Caspase11-KO InvivoGen Cat.code: raw-kocasp11 RAW-ASC-GSDMD-KO InvivoGen Cat.code: raw-kogsdmd HEK293T ATCC CRL-3216 AD293 Agilent Technologies #240085 HeLa JCRB Cell Bank JCRB9004 Recombinant DNA pET-BA BioDynamics Laboratory Inc. DV210 Halotag ORF Clone pFN21AE1334 Kazusa DNA Research Institute FHC08921 Software and algorithms ImageJ Schneider et al. 32 https://imagej.nih.gov/ij/ GraphPad Prism 10 GraphPad https://www.graphpad.com/features Flowjo BD https://www.flowjo.com/ BioRender BioRender https://www.biorender.com/ AutoDock Vina Version 1.2.7 The Center for Computational Structural Biology (CCSB) https://vina.scripps.edu/ PyMOL DeLano Scientific LLC https://www.pymol.org/ 18 Cell Reports 44, 116568, December 23, 2025 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL Macrophage colony stimulating factor (M-CSF) (BioLegend) to differentiate into BMDM. ..
Lactate Dehydrogenase Assay:Article Title: Modulation function of sphingomyelin molecular species in TLR4 signaling and cell death.
Article Snippet: The obtained bone marrow cells were washed in DMEM and cultured for 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL recombinant mouse Granulocyte-macrophage colony-stimulating factor (GM-CSF) (BioLegend) and IL4 (BioLegend) to differentiate into BMDC or, cultured for .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays ELISA MAX Deluxe Set Mouse IL-1α BioLegend 433404 ELISA MAX Deluxe Set Mouse IL-1β BioLegend 432604 Mouse IL-6 ELISA Kit Invitrogen BMS603-2 Human IL-6 ELISA Kit Invitrogen EH2IL6 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3TEN Pierce TM BCA Protein Assay Kits Thermo Fisher Scientific 23227 Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs Bio-Rad 1704156 Cell Counting Kit-8 Dojindo CK04 Cytotoxicity LDH Assay Kit-WST Dojindo CK12 Experimental models: Cell lines Raw264.7 ATCC TIB-71 THP-1 JCRB Cell Bank JCRB0112 HEK-Blue TM hTLR4 InvivoGen Cat.code: hkb-htlr4 HEK-Blue TM mTLR4 InvivoGen Cat.code: hkb-htlr4 RAW-ASC-WT InvivoGen Cat.code: raw-asc RAW-ASC-Caspase11-KO InvivoGen Cat.code: raw-kocasp11 RAW-ASC-GSDMD-KO InvivoGen Cat.code: raw-kogsdmd HEK293T ATCC CRL-3216 AD293 Agilent Technologies #240085 HeLa JCRB Cell Bank JCRB9004 Recombinant DNA pET-BA BioDynamics Laboratory Inc. DV210 Halotag ORF Clone pFN21AE1334 Kazusa DNA Research Institute FHC08921 Software and algorithms ImageJ Schneider et al. 32 https://imagej.nih.gov/ij/ GraphPad Prism 10 GraphPad https://www.graphpad.com/features Flowjo BD https://www.flowjo.com/ BioRender BioRender https://www.biorender.com/ AutoDock Vina Version 1.2.7 The Center for Computational Structural Biology (CCSB) https://vina.scripps.edu/ PyMOL DeLano Scientific LLC https://www.pymol.org/ 18 Cell Reports 44, 116568, December 23, 2025 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL Macrophage colony stimulating factor (M-CSF) (BioLegend) to differentiate into BMDM. ..
Recombinant:Article Title: Modulation function of sphingomyelin molecular species in TLR4 signaling and cell death.
Article Snippet: The obtained bone marrow cells were washed in DMEM and cultured for 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL recombinant mouse Granulocyte-macrophage colony-stimulating factor (GM-CSF) (BioLegend) and IL4 (BioLegend) to differentiate into BMDC or, cultured for .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays ELISA MAX Deluxe Set Mouse IL-1α BioLegend 433404 ELISA MAX Deluxe Set Mouse IL-1β BioLegend 432604 Mouse IL-6 ELISA Kit Invitrogen BMS603-2 Human IL-6 ELISA Kit Invitrogen EH2IL6 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3TEN Pierce TM BCA Protein Assay Kits Thermo Fisher Scientific 23227 Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs Bio-Rad 1704156 Cell Counting Kit-8 Dojindo CK04 Cytotoxicity LDH Assay Kit-WST Dojindo CK12 Experimental models: Cell lines Raw264.7 ATCC TIB-71 THP-1 JCRB Cell Bank JCRB0112 HEK-Blue TM hTLR4 InvivoGen Cat.code: hkb-htlr4 HEK-Blue TM mTLR4 InvivoGen Cat.code: hkb-htlr4 RAW-ASC-WT InvivoGen Cat.code: raw-asc RAW-ASC-Caspase11-KO InvivoGen Cat.code: raw-kocasp11 RAW-ASC-GSDMD-KO InvivoGen Cat.code: raw-kogsdmd HEK293T ATCC CRL-3216 AD293 Agilent Technologies #240085 HeLa JCRB Cell Bank JCRB9004 Recombinant DNA pET-BA BioDynamics Laboratory Inc. DV210 Halotag ORF Clone pFN21AE1334 Kazusa DNA Research Institute FHC08921 Software and algorithms ImageJ Schneider et al. 32 https://imagej.nih.gov/ij/ GraphPad Prism 10 GraphPad https://www.graphpad.com/features Flowjo BD https://www.flowjo.com/ BioRender BioRender https://www.biorender.com/ AutoDock Vina Version 1.2.7 The Center for Computational Structural Biology (CCSB) https://vina.scripps.edu/ PyMOL DeLano Scientific LLC https://www.pymol.org/ 18 Cell Reports 44, 116568, December 23, 2025 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL Macrophage colony stimulating factor (M-CSF) (BioLegend) to differentiate into BMDM. ..
Software:Article Title: Modulation function of sphingomyelin molecular species in TLR4 signaling and cell death.
Article Snippet: The obtained bone marrow cells were washed in DMEM and cultured for 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL recombinant mouse Granulocyte-macrophage colony-stimulating factor (GM-CSF) (BioLegend) and IL4 (BioLegend) to differentiate into BMDC or, cultured for .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays ELISA MAX Deluxe Set Mouse IL-1α BioLegend 433404 ELISA MAX Deluxe Set Mouse IL-1β BioLegend 432604 Mouse IL-6 ELISA Kit Invitrogen BMS603-2 Human IL-6 ELISA Kit Invitrogen EH2IL6 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3TEN Pierce TM BCA Protein Assay Kits Thermo Fisher Scientific 23227 Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs Bio-Rad 1704156 Cell Counting Kit-8 Dojindo CK04 Cytotoxicity LDH Assay Kit-WST Dojindo CK12 Experimental models: Cell lines Raw264.7 ATCC TIB-71 THP-1 JCRB Cell Bank JCRB0112 HEK-Blue TM hTLR4 InvivoGen Cat.code: hkb-htlr4 HEK-Blue TM mTLR4 InvivoGen Cat.code: hkb-htlr4 RAW-ASC-WT InvivoGen Cat.code: raw-asc RAW-ASC-Caspase11-KO InvivoGen Cat.code: raw-kocasp11 RAW-ASC-GSDMD-KO InvivoGen Cat.code: raw-kogsdmd HEK293T ATCC CRL-3216 AD293 Agilent Technologies #240085 HeLa JCRB Cell Bank JCRB9004 Recombinant DNA pET-BA BioDynamics Laboratory Inc. DV210 Halotag ORF Clone pFN21AE1334 Kazusa DNA Research Institute FHC08921 Software and algorithms ImageJ Schneider et al. 32 https://imagej.nih.gov/ij/ GraphPad Prism 10 GraphPad https://www.graphpad.com/features Flowjo BD https://www.flowjo.com/ BioRender BioRender https://www.biorender.com/ AutoDock Vina Version 1.2.7 The Center for Computational Structural Biology (CCSB) https://vina.scripps.edu/ PyMOL DeLano Scientific LLC https://www.pymol.org/ 18 Cell Reports 44, 116568, December 23, 2025 7 d in DMEM containing 10% (v/v) FBS and 40 ng/mL Macrophage colony stimulating factor (M-CSF) (BioLegend) to differentiate into BMDM. ..
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